rat astrocyte media  (Cell Applications Inc)

Journal: Cells

Article Title: Microfluidic Model to Evaluate Astrocyte Activation in Penumbral Region following Ischemic Stroke

doi: 10.3390/cells11152356

Figure Lengend Snippet: Cell viability analysis at varying densities within polystyrene and PDMS microfluidic devices. ( A ) Schematic representation of polystyrene and PDMS microfluidic devices. Both devices had one lumen located in the culture chamber flank, creating an asymmetric oxygen and nutrient distribution. Since PDMS is permeable to oxygen, only a gradient of nutrient is created in the PDMS-based microdevice. ( B ) Fluorescence microscopy image of astrocytes within polystyrene microfluidic device at 5 million cells/mL. Live and dead cells are shown in green and red, respectively. The location of the lumen is shown in a dashed line. ( C ) Astrocytes within PDMS microfluidic devices at varying densities imaged at lumen and necrotic core. Red and blue backgrounds are used to denote results obtained in polystyrene and PDMS devices respectively. Green and red outlines are used to denote results obtained from the proximal and distal region respectively. ( D ) Astrocytes within polystyrene microfluidic devices at varying densities imaged at lumen and necrotic core. ( E ) Graphs depicting cellular viability of astrocytes at densities of 3 million/mL and 5 Million/mL within polystyrene and PDMS microfluidic devices. *, **, *** denote p -value < 0.05, 0.01, 0.005 respectively.

Article Snippet: Rat astrocytes were cultured in rat astrocyte basal medium containing growth serum (Cell Applications, Cat: R820-485, Lot: 160).

Techniques: Fluorescence, Microscopy

Journal: Cells

Article Title: Microfluidic Model to Evaluate Astrocyte Activation in Penumbral Region following Ischemic Stroke

doi: 10.3390/cells11152356

Figure Lengend Snippet: Experimental Design. Illustration depicting experimental timeline beginning with the harvesting of rat astrocytes and culture within microfluidic device at different time periods followed by the extraction of astrocytes from device and culture of astrocytes prior to calcium signaling analysis and morphological analysis.

Article Snippet: Rat astrocytes were cultured in rat astrocyte basal medium containing growth serum (Cell Applications, Cat: R820-485, Lot: 160).

Techniques: Extraction

Journal: Cells

Article Title: Microfluidic Model to Evaluate Astrocyte Activation in Penumbral Region following Ischemic Stroke

doi: 10.3390/cells11152356

Figure Lengend Snippet: Calcium signaling analysis. ( A ) Illustration depicting analysis of calcium waves generated by astrocyte signaling ( B ) Image depicting astrocytes stained with the calcium-sensing dye. Red outline highlights a pulsing astrocyte ( C ) Graph illustrating differing types of astrocyte calcium pulsing ( D ) Graphs analyzing percentage of pulsing astrocytes from device illustrating differences at proximal and distal locations at varying time points ( E ) Graphs analyzing percentage of pulsing astrocytes from device illustrating recovery potential of astrocytes at proximal and distal locations. *, **, *** denote p -value < 0.05, 0.01, 0.001 respectively.

Article Snippet: Rat astrocytes were cultured in rat astrocyte basal medium containing growth serum (Cell Applications, Cat: R820-485, Lot: 160).

Techniques: Generated, Staining

Journal: Cells

Article Title: Microfluidic Model to Evaluate Astrocyte Activation in Penumbral Region following Ischemic Stroke

doi: 10.3390/cells11152356

Figure Lengend Snippet: Morphological Analysis. ( A ) Illustration depicting methodology for circularity morphological analysis. ( B ) Astrocytes extracted from the proximal region of the device after 3 days and placed in culture for 5 days prior to imaging. ( C ) Astrocytes extracted from the distal region of the device after 3 days and placed in culture for 5 days prior to imaging. ( D ) Violin graphs analyzing circularity of astrocytes at multiple time points after extraction from proximal and distal regions of the device. ( E ) Violin graphs analyzing area of astrocytes at multiple time points after extraction from proximal and distal regions of the device. ** and *** denote p -value < 0.01 and 0.005 respectively.

Article Snippet: Rat astrocytes were cultured in rat astrocyte basal medium containing growth serum (Cell Applications, Cat: R820-485, Lot: 160).

Techniques: Imaging, Extraction